DMSO Calculator

Calculate accurate DMSO dilutions

⚠️ Important Safety Information:

  • Consult healthcare professionals before using DMSO
  • Only use pharmaceutical/medical grade DMSO
  • Incorrect dilution can be dangerous
  • This calculator is for reference only

ℹ️ Pure DMSO Properties:

  • Density: 1.1 g/mL at room temperature (20°C)
  • Freezing point: 18.5°C (solidifies near room temperature)


For small volumes, enter as decimal (e.g., 0.5 for half a mL)

Please enter a valid volume greater than 0



Enter the concentration of your DMSO stock solution

Must be between 0-100%



Enter your desired final DMSO concentration

Must be less than starting concentration

💡 Common Dilutions (click to auto-fill)


About DMSO & FAQs

Dimethyl sulfoxide (DMSO), a polar aprotic solvent, emerged in 1866 from lignin in wood pulping—a byproduct of the pulp and paper industry. This naturally sourced compound dissolves a wide array of substances and penetrates tissues and materials effectively. It finds common use in chemical extractions, as a versatile carrier, for therapeutic purposes in medical contexts, and in preserving tissues via cryopreservation.

Why might someone dilute DMSO?
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Dilution can adjust concentration for specific purposes, such as making it easier to handle or combine with other substances, but always check with a professional for your intended use.

What factors should be considered when working with DMSO?
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Key considerations include using high-purity grades, accurate measurements, and proper safety equipment; compatibility with containers and other materials is also important.

How should I store diluted DMSO?
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In glass bottles, away from light and heat, at room temperature; use within a few months for best efficacy.

Where can I learn more about DMSO?
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For additional insights into its history and potential uses, explore A Midwestern Doctor’s series on Substack: The Forgotten Side of Medicine. Always consult professionals for guidance.

References

  • Jacob, S. W., & Wood, D. C. (1971). Dimethyl sulfoxide (DMSO) toxicology, pharmacology, and clinical experience. American Journal of Surgery, 114(3), 414-426.
  • Santos, N. C., et al. (2003). Multidisciplinary utilization of dimethyl sulfoxide: pharmacological, cellular, and molecular aspects. Biochemical Pharmacology, 65(7), 1035-1041.
  • LeBel, R. G., & Goring, D. A. I. (1962). Density, viscosity, refractive index, and hygroscopicity of mixtures of water and dimethyl sulfoxide. Journal of Chemical & Engineering Data, 7(1), 100-101.
  • Cowie, J. M. G., & Toporowski, P. M. (1961). Association in the binary liquid system dimethyl sulphoxide-water. Canadian Journal of Chemistry, 39(11), 2240-2243.